Detection of α 1-Type-I Collagen Gene Expression in Patellar Tendon Using Competitive Reverse Transcribed Polymerase Chain Reaction 竞争性反转录聚合酶链反应对髌韧带中的α1-Ⅰ型胶原蛋白基因表达的定量检测
Methods Tendon injury and adhesive tissue models were made in zone ⅱ of the long toes of 20 Leghorn chicken. The mRNA from the tendon and adhesive tissue harvested eight weeks after operation was used for reverse transcription-polymerase chain reaction ( RT-PCR). 方法取20只Leghorn鸡,制作肌腱损伤粘连模型,术后8周分别取损伤部位肌腱、粘连组织,提取mRNA,进行逆转录-聚合酶链反应和电泳。